Biochem/physiol Actions

ED₅₀ = 0.125-0.5 µg/ml as measured by inhibition of the biological activity of IGF-I or IGF-II (30 ng/ml) on MCF-7 human breast adenocarcinoma cells.

General description

Recombinant, mouse insulin-like growth factor binding protein 3 (IGFBP-3) consisting of amino acids 22-291 and expressed in NS0-derived murine myeloma cells with four substitutions: Arg250Gln, Gln259Arg, Ser260Gly, Arg271Pro. Mouse IGFBP-3 cDNA encodes a 291 amino acid residue precursor protein with a putative 27 aa residue signal peptide.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Schuller, A.G.P., et al. 1994. Mol. Cell. Endoc.104, 57.

Packaging

25 µg in Glass bottle

Physical form

Lyophilized from 0.2 µm filtered Acetonitrile, TFA.

Reconstitution

Reconstitute to 100 µg/ml in sterile PBS.

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)